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  文件名称:  Oxfordbiomed URINARY ISOPROSTANE EIA KIT, 5 PACK $1,270.00 SKU EA85.5
  公司名称:  上海拜力生物科技有限公司
  下载次数:   32
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Oxfordbiomed URINARY ISOPROSTANE EIA KIT, 5 PACK $1,270.00 SKU EA85.5

URINARY ISOPROSTANE EIA KIT, 5 PACK

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ASSAY PRINCIPLE

北京赛车pk10代理 This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 15-F2t-Isoprostane (the best characterized isoprostane) in urine samples. Briefly, urine samples are mixed with an enhanced dilution buffer that essentially eliminates interference due to non-specific binding. The 15-F2t-Isoprostane in the samples or standards competes with 15-F2t-Isoprostane conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 15-F2t-Isoprostane coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-F2t-Isoprostane-HRP bound and inversely proportional to the amount of unconjugated 15-F2t-Isoprostane in the samples or standards.

TYPICAL STANDARD CURVE

RECOMMENDED PRODUCT

When using urine as a sample, it is recommended to run our Creatinine Assay Kit () in conjunction with your samples since urinary creatinine levels may be used to normalize the rate of excretion of other analytes.
 

RECOMMENDED REFERENCES

1. Morrow, J., Harris, T., & Roberts, L., Anal. Biochem. 14:1-10 (1990).
2. Morrow, J. D., Hill, K. , Burke, R. F., Nammour, T. M., Badr, K. F., and Roberts, L. J., Proc. Natl. Acad. Sci. U.S.A. 87:9383-9387 (1990).
3. Morrow, et. al., The Isoprostanes: Unique Bioactive Products of Lipid Peroxidation. Prog. Lipid Res. 36:1-21, 1997.
4. Morrow, J., Awad, J. A., Boss, H. J., Blair, I. A., and Roberts, L. J., Proc. Natl Acad. Sci USA 89:10721-10725 (1992).
5. Wang, et al., Immunological characterization of urinary 8-epi-prostaglandin F2a excretion in man. J. Pharm. Exp. Ther. 275:94-100 (1995).
6. Morrow, J., Zackert, W., Yang, J., Kuhrts, E., Callewaert, D., Taber, D., Oates, J., Roberts, J., Quantitiation of the major urinary metabolite of the isoprostane 15-F2t-Isoprostane(8-iso-PGF2a) by a stable isotope dilution mass spectrometric assay, Analytical Biochem. 269: 326-331 (1999).
北京赛车pk10代理 7. Roberts II, L.J., Morrow, J.D., Measurement of F2-isoprostanes as an index of oxidative stress in vivo, Free Radic. Biol. Med. 28:505-513 (2000).

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